Clear your cookies and try again, and see if you can reduce the size and amount of cookies your app is using.
When you set the registry key value you make sure you consider the below points:
1) Calculate the size of the user's Kerberos token by using the formula that's described in the following Knowledge Base article:
327825 Problems with Kerberos authentication when a user belongs to many groups
2) Set the value of MaxFieldLength and MaxRequestBytes on the server to 4/3 * T, where T is the user's token size in bytes. HTTP encodes the Kerberos token by using base64 encoding.
https://support.microsoft.com/en-us/help/2020943/http-400-bad-request-request-header-too-long-response-to-http-request
Note: Make sure you restarted the machine after doing changes.
you could also try to add below code in your site web.config file:
<configuration>
<system.webServer>
<security>
<requestFiltering>
<requestLimits maxAllowedContentLength="500000000" />
</requestFiltering>
</security>
<system.webServer>
<system.web>
<httpRuntime maxRequestLength="500000000" executionTimeout="120" />
</system.web>
</configuration>
if you still face same issue try to use the fiddler or any other tool to capture network traffic and properly analyze the request and response header.