2
votes

I'm working with a dataset on conservation and its influence on biomass, in which fifty plots of land, each one hectare, were sampled at random from a ten thousand hectare area in Northern England.

For each plot of land, the following variables were recorded:

• biomass: an estimate of the biomass of vegetation in kg per square metre.

• alt: the mean altitude of the plot in metres above sea level.

• cons: a categorical variable, which was coded 1 if the plot was part of a conservation area, and 2 otherwise.

• soil a categorical variable crudely classifying soil type as 1 for chalk, 2 for clay and 3 for loam.


At the moment I am struggling with two things in particular:

How to calculate the average difference in biomass between clay (soil2) and loam (soil3) soils based on my fitted model (model1) and calculate 95% confidence interval for this mean predicted value.

And how to calculate the mean predicted biomass for a plot located in a conservation area with predominantly clay soil at an altitude of 300m?

This is a summary of the linear model that I'm working with.

Call:
lm(formula = biomass ~ alt + soil + cons, data = conservation)

Residuals:
      Min        1Q    Median        3Q       Max 
-0.183105 -0.052926  0.005593  0.061844  0.194402 

Coefficients:
              Estimate Std. Error t value Pr(>|t|)    
(Intercept)  2.2928629  0.0357850  64.073  < 2e-16 ***
alt         -0.0029068  0.0001302 -22.318  < 2e-16 ***
soil2       -0.0862220  0.0342955  -2.514   0.0156 *  
soil3       -0.2309939  0.0354480  -6.516 5.33e-08 ***
cons2        0.0488634  0.0292075   1.673   0.1013    
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Residual standard error: 0.09428 on 45 degrees of freedom
Multiple R-squared:  0.9459,    Adjusted R-squared:  0.9411 
F-statistic: 196.7 on 4 and 45 DF,  p-value: < 2.2e-16

And here's the data:

dput(conservation)

structure(list(biomass = c(2.01, 2.06, 1.7, 2.07, 1.88, 2.11, 
0.98, 2.14, 1.75, 1.81, 2.15, 1.68, 2.23, 2.04, 1.67, 1.77, 1.74, 
1.53, 1.79, 2.15, 1.39, 2.19, 2.14, 2.29, 1.91, 1.73, 2.21, 1.96, 
2.07, 2.01, 2.2, 2.24, 1.33, 1.05, 1.36, 1.72, 1.44, 1.52, 2.09, 
1.42, 1.64, 0.92, 1.65, 1.37, 0.77, 1.57, 2.25, 2.23, 2.03, 1.18
), alt = c(116L, 21L, 130L, 65L, 117L, 82L, 359L, 5L, 86L, 91L, 
64L, 178L, 79L, 70L, 209L, 110L, 161L, 248L, 146L, 23L, 237L, 
84L, 40L, 7L, 161L, 122L, 25L, 146L, 67L, 118L, 42L, 57L, 277L, 
338L, 331L, 153L, 239L, 237L, 67L, 171L, 206L, 371L, 107L, 236L, 
482L, 240L, 56L, 42L, 68L, 436L), cons = structure(c(2L, 2L, 
1L, 2L, 2L, 1L, 1L, 2L, 2L, 1L, 2L, 2L, 2L, 2L, 1L, 1L, 2L, 1L, 
1L, 2L, 2L, 2L, 1L, 2L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 1L, 
1L, 1L, 2L, 2L, 1L, 1L, 2L, 2L, 1L, 2L, 1L, 2L, 2L, 2L, 2L, 2L
), .Label = c("1", "2"), class = "factor"), soil = structure(c(2L, 
3L, 2L, 2L, 2L, 1L, 2L, 2L, 3L, 3L, 2L, 1L, 1L, 2L, 2L, 3L, 2L, 
3L, 2L, 2L, 3L, 1L, 3L, 2L, 1L, 3L, 1L, 1L, 1L, 1L, 1L, 1L, 3L, 
3L, 2L, 1L, 3L, 2L, 1L, 3L, 3L, 3L, 3L, 3L, 2L, 2L, 1L, 1L, 1L, 
2L), .Label = c("1", "2", "3"), class = "factor"), alt.factor = 
structure(c(1L, 
1L, 2L, 1L, 1L, 1L, 2L, 1L, 1L, 1L, 1L, 2L, 1L, 1L, 2L, 1L, 2L, 
2L, 2L, 1L, 2L, 1L, 1L, 1L, 2L, 2L, 1L, 2L, 1L, 2L, 1L, 1L, 2L, 
2L, 2L, 2L, 2L, 2L, 1L, 2L, 2L, 2L, 1L, 2L, 2L, 2L, 1L, 1L, 1L, 
2L), .Label = c("below median", "above median"), class = "factor")), 
.Names = c("biomass", 
"alt", "cons", "soil", "alt.factor"), row.names = c(NA, -50L), class = 
"data.frame")

1
Dude, you're basically asking people to do your homework for you. C'mon.ulfelder
@ulfelder Don't worry. People like to do that.Bhargav Rao

1 Answers

3
votes

How to calculate the average difference in biomass between clay (soil2) and loam (soil3) soils based on my fitted model (model1) and calculate 95% confidence interval for this mean predicted value.

Strictly speaking, this is a special case of something we call "linear hypothesis test". But I think it is not the intention of your assignment, so I will not go for this approach. If you are interested in it, read Get p-value for group mean difference without refitting linear model with a new reference level.

What I will do here, is to simply use a different factor level as contrast and refit your model. At the moment, you have "soil1" as contrast level; I will reset "soil2" as contrast level. Have a look at How to set contrasts for my variable in regression analysis with R? for a general treatment.

fit <- lm(biomass ~ alt + soil + cons, data = conservation,
          contrasts = list(soil = contr.treatment(n = 3, base = 2)))

#Coefficients:
#              Estimate Std. Error t value Pr(>|t|)    
#(Intercept)  2.2066409  0.0400572  55.087  < 2e-16 ***
#alt         -0.0029068  0.0001302 -22.318  < 2e-16 ***
#soil1        0.0862220  0.0342955   2.514   0.0156 *  
#soil3       -0.1447719  0.0325295  -4.450 5.59e-05 ***
#cons2        0.0488634  0.0292075   1.673   0.1013    
#---
#Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
#
#Residual standard error: 0.09428 on 45 degrees of freedom
#Multiple R-squared:  0.9459,   Adjusted R-squared:  0.9411 
#F-statistic: 196.7 on 4 and 45 DF,  p-value: < 2.2e-16

Now, the coefficient for "soil3" is giving the difference of group mean for "soil3" and the contrast level "soil2". It is pretty straightforward to obtain confidence interval for this coefficient, from the standard error and residual degree of freedom of the model, but again, that might be too technical to you. Consider using confint:

confint(fit, "soil3", level = 0.95)
#           2.5 %     97.5 %
#soil3 -0.2102896 -0.0792541

And how to calculate the mean predicted biomass for a plot located in a conservation area with predominantly clay soil at an altitude of 300m?

For prediction on response biomass, we can use predict:

predict(fit, newdata = list(alt = 300, soil = "2", cons = "1"))
#       1 
#1.334606 

So the prediction mean is about 1.3346.