I am new to MATLAB Image Processing and currently I have two images - one is a grayscale image of my object and the second is the scaled image generated from MATLAB using imagesc function. I am trying to overlay this scaled image on top of my grayscale image to get a spatial resolution for easier observation. Attached are the two images:
A) Grayscale Image:
B) Scaled Image:
There were a few difficulties that I encountered. Firstly, the scaled image is not saved in the same pixel dimensions, but I can get around that using the imwrite function:
im = imagesc(ScaledDiff);
imwrite(get(im,'cdata'),'scaleddiff.tif')
However, doing so will result in a loss of colorbar and the colormap. Secondly, even if I manage to shrink the scaled image to the size of the grayscale image, overlaying it is still a challenge. Ideally, I would like to set the transparency (or 'alpha') to 0 for those pixels with < 0.02 in scaled image value.
Any idea on how to do this will be greatly appreciated! Sorry if I was unclear!
UPDATE: Thanks to Rotem, I have managed to overlay the grayscale image and a particular region of my heatmap:
However, I need to display the colorbar corresponding to the heatmap values, because otherwise the information is lost and the overlay will be useless. How should I do this? Below is a snippet of my code, where ScaledIntDiff contains the values from 0 to 0.25 that is displayed on the heatmap:
Brightfield = imread('gray.jpg'); % read background image
I1 = ind2rgb(gray2ind(Brightfield), gray); % convert indices into RGB scale
scale = 1000;
ScaledIntDiff2 = round(ScaledIntDiff.*scale);
I2 = ind2rgb(ScaledIntDiff2, jet(256)); % this is for the heatmap
threshold = 0.02;
I2R = I2(:,:,1); I2G = I2(:,:,2); I2B = I2(:,:,3);
I1R = I1(:,:,1); I1G = I1(:,:,2); I1B = I1(:,:,3);
% Replace pixels in I2 with pixels in I1 if the value of ScaledIntDiff of those pixels is below the threshold
I2R(ScaledIntDiff<threshold) = I1R([ScaledIntDiff<threshold]);
I2G(ScaledIntDiff<threshold) = I1G([ScaledIntDiff<threshold]);
I2B(ScaledIntDiff<threshold) = I1B([ScaledIntDiff<threshold]);
I2(:,:,1) = I2R; I2(:,:,2) = I2G; I2(:,:,3) = I2B;
figure
imshow(I2)
I know that the code above is highly inefficient, so suggestions on how to improve it will be very welcomed. Thank you!
imagesc
, create RGB image usingind2rgb
. Example:I = imread('cameraman.tif');J = ind2rgb(I, parula(256));imshow(J)
. – Rotemimresize
. – Rotem